Ten years after the first American Pie movie, three new hapless virgins discover the Bible hidden in the school library at East Great Falls High. Unfortunately for them, the book is ruined, and with incomplete advice, the Bible leads them on a hilarious journey to lose their virginity.
Christine (Jennifer Holland) and Reagan (Kate Plec) are a pair of young women who get to know each other when they end up sharing a dorm room in college. Christine is a studious type who is still getting over a family tragedy, while Reagan never lets her classes get in the way of a good time. Convinced Christine needs to get out more, Reagan persuades her to tag along as she attends a wild party at the Beta Alpha Tau sorority house. Both Christine and Reagan are impressed with the BAT house, and in particular, chapter president Devin (Michelle Borth), and they are soon invited to join. However, Ms. Masters (Barbara Crampton), who teaches a class on the paranormal, believes that the BAT house is the center of supernatural activity on campus, and urges Christine to inform her of the goings-on at the house. It isn’t long before Christine finds out that Ms. Masters’ fears were based in fact, but can she tear herself away from the decadent pleasures sorority life has brought to her?
In the not too distant future a secret government re-animation chemo-virus gets released into conservative Sartre, Nebraska and lands in an underground strip club. As the virus begins to spread, turning the strippers into “Super Zombie Strippers” the girls struggle with whether or not to conform to the new “fad” even if it means there’s no turning back.